PSORALEN PHOTOLESIONS IN THE PHOSPHOLIPIDS.
Zofia Zarebska1, Ewa Waszkowska1, Sergio Caffieri2, Francesco Dall'Acqua2
1Institute of Biochemistry and Biophysics PAS, 02-106 Warszawa, Poland;
2Institute of Pharmaceutical Sciences,Centro del CNR, 35131 Padova, Italy
The formation of d1<>PSO cyclobutane adducts in the model lecithins was shown. The adducts are formed in the both, hydrophylic and hydrophobic media with approximately 5% yield at the pharmacological doses of UVA radiation.
The d1<>PSO and d1<>pyrone adducts identified by absorption and NMR spectroscopy can serve as markers of the phospholipid lesions in the cell.
It was anticipated that photochemically induced modifications: psoralen photoaddition to the vinyl bonds of fatty acids, fatty acid-adducts release from the lecithin and trans-isomerisation around vinyl bonds - altogether can affect the membrane fluidity and the recognition processes of the membrane receptors .