Figure 4. Comparison of amino acid sequences of Arabidopsis and E. coli FPGs.
1 60
|<---------------------------Exon 1------------------------------
AtFPG-4 MPELPEVEAA RRAIEENCLG KKIKRVIIAD DNKVIHGISP SDFQTSILGK TIISARRKGK
AtFPG-3 MPELPEVEAA RRAIEENCLG KKIKRVIIAD DNKVIHGISP SDFQTSILGK TIISARRKGK
AtFPG-1 MPELPEVEAA RRAIEENCLG KKIKRVIIAD DNKVIHGISP SDFQTSILGK TIISARRKGK
AtFPG-2 MPELPEVEAA RRAIEENCLG KKIKRVIIAD DNKVIHGISP SDFQTSILGK TIISARRKGK
E.coliFPG MPELPEVETS RRGIEPHLVG ATILHAVVR. NGRLRWPVSE EIYRLS..DQ PVLSVQRRAK
Consensus MPELPEVE-- RR-IE----G --I------- --------S- -----S---- ---S--R--K
61 120
---------------->|<------Exon 2----->|<-------Exon 3----->|<-----
AtFPG-4 NLWLELDSPP FPSFQFGMAG AIYIKGVAVT KYKRSAVKDS EEWPSKYSKF FVELDDGLEL
AtFPG-3 NLWLELDSPP FPSFQFGMAG AIYIKGVAVT KYKRSAVKDS EEWPSKYSKF FVE.......
AtFPG-1 NLWLELDSPP FPSFQFGMAG AIYIKGVAVT KYKRSAVKDS EEWPSKYSKF FVELDDGLEL
AtFPG-2 NLWLELDSPP FPSFQFGMAG AIYIKGVAVT KYKRSAVKDS EEWPSKYSKF FVELDDGLEL
E.coliFPG YLLLELPE.G WIIIHLGMSG SL........ .....RILPE ELPPEKHDHV DLVMSNGKVL
Consensus -L-LEL---- ------GM-G ---------- ---------- E--P-K---- ----------
121 180
---Exon 4------->|<-----------------Exon 5-------------------->|<
AtFPG-4 SFTDKRRFAK VRLLANPTSV SPISELGPDA LLEPMTVDEF AESLAKKKIT IKPLLLDQGY
AtFPG-3 .......... ......PTSV SPISELGPDA LLEPMTVDEF AESLAKKKIT IKPLLLDQGY
AtFPG-1 SFTDKRRFAK VRLLANPTSV SPISELGPDA LLEPMTVDEF AESLAKKKIT IKPLLLDQGY
AtFPG-2 SFTDKRRFAK VRLLANPTSV SPISELGPDA LLEPMTVDEF AESLAKKKIT IKPLLLDQGY
E.coliFPG RYTDPRRFGA WLWTKELEGH NVLTHLGPEP LSDDFNGEYL HQKCAKKKTA IKPWLMDNKL
Consensus ---------- ---------- -----LGP-- ---------- ----AKKK-- IKP-L-D---
181 240
--- Exon 6----->|<----------Exon 7---------->|<------------------
AtFPG-4 ISGIGNWIAD EVLYQARIHP LQTASSLSKE QCEALHTSIK EVIQHAVQVN ADS.KEF...
AtFPG-3 ISGIGNWIAD EVLYQARIHP LQTASSLSKE QCESLHTSIK EVIQHAVQVN ADS.KEF...
AtFPG-1 ISGIGNWIAD EVLYQARIHP LQTASSLSKE QCEALHTSIK EVIEKAVEVD ADS.SQF...
AtFPG-2 ISGIGNWIAD EVLYQARIHP LQTASSLSKE QCEALHTSIK EVIQHAVQVN ADS.KEF...
E.coliFPG VVGVGNIYAS ESLFAAGIHP DRLASSLSLA ECELLARVIK AVLLRSIEQG GTTLKDFLQS
Consensus --G-GN--A- E-L--A-IHP ---ASSLS-- -CE-L---IK -V-------- ------F---
241 300
----Exon 8-13------------------------>|<----------------------
AtFPG-4 ...PVEWLFH FR.WGKKAGK ..VNGKKIDF ITAG..GRTT AYVPELQKLY GKDAEKAAKV
AtFPG-3 ...PVEWLFH FR.WGKKAGK ..VNGKKIDF ITAG..GRTT AYVPELQKLY GKDAEKAAKV
AtFPG-1 ...PSNWIFH NR..EKKPGK AFVDGKKIDF ITAG..GRTT AYVPELQKLY GKDAEKAAKV
AtFPG-2 ...PVEWLFH FR.WGKKAGK ..VNGKLSHH LSINLMKQNL GFCR~~~~~~ ~~~~~~~~~~
E.coliFPG DGKPGYFAQE LQVYGRKGEP CRVCGTPI.. VATKHAQRAT FYCRQCQK~~ ~~~~~~~~~~
Consensus ---P------ ------K--- --V-G----- ---------- ---------- ----------
301 360
----------------------------Exon 14------------------------------
AtFPG-4 RPAKRGVKPK EDDGDGEEDE ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~
AtFPG-3 RPAKRGVKPK EDDGDGEEDE QETEKEDESA KSKKGQKPRG GRGKKPASKT KTEESDDDGD
AtFPG-1 RPAKRGVKPK EDDGDGEEDE QETEKEDESA KSKKGQKPRG GRGKKPASKT KTEESDDDGD
AtFPG-2 ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~
E.coliFPG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~
Consensus ---------- ---------- ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~
351 391
-------------------------------------------->|
AtFPG-4 ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~
AtFPG-3 DSEAEEEVVK PKGRGTKPAI KRKSEEKATS QAGKKPKGRK S*
AtFPG-1 DSEAEEEVVK PKGRGTKPAI KRKSEEKATS QAGKKPKGRK S~
AtFPG-2 ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~
E.coliFPG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~
Consensus ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~